An experimental study with 22 ponies was conducted to investigate the effects of feeding a diet high in nonstructural carbohydrates (starch) on the laminae of lean ponies compared with obese ponies. The ponies were sorted into lean (body condition ≤4) and obese (body condition ≥7) groups and half of each group was fed either a low starch hay diet or a high starch diet with added sweet feed and plant sugars. After 7 days, laminar tissue samples were collected and analyzed to measure the activity of naturally occurring substances that trigger inflammation (inflammatory mediators) and the expression of genes that control inflammation.